اثر اسانس آویشن شیرازی بر تولید یون سوپراکسید و نیتریک اکسید و بیان ژن‌های NADH اکسیداز و نیتریک اکسید سنتاز در سلول‌های ماکروفاژ

Objective: In this study, inhibitory effect of Zataria multiflora essential oil on superoxide and nitric oxide production and NADH oxidase (NOX) and nitric oxide synthase (NOS) expression was examined. Materials and Methods: Zataria essential oil was obtained by hydro-distillation and chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). Superoxide and nitric oxide scavenging effects of zataria essential oil were tested by biochemical assays. Then, LPS-stimulated macrophages were treated by different concentrations of essential oil and expression of superoxide-producing enzyme and nitric oxide-producing enzyme was investigated by Real-time PCR, also superoxide anion and nitric oxide levels was examined by biochemical assays. Results: The main components of the zataria essential oil were linalool (32.9%), carvacrol (26.7%), thymol (12.5%), p-Cymene (4.5%) and α-Pinene (2%). Zataria essential oil sequestered superoxide anion (O2-) and nitric oxide (NO) in in vitro assay at low concentrations of 70-100 µg/ml. Zataria essential oil at non-cytotoxic level strongly reduced intracellular reactive oxygen species (ROS) and nitric oxide (NO) production in LPS-stimulated macrophages. Superoxide-producing enzyme and NO producing enzyme expression in LPS-stimulated murine macrophage (cell line J774) was declined by zataria essential oil. Conclusion: Due to the results, the in vivo antioxidant activity of zataria essential oil may be attributed to down-production of NO and superoxide and also down-regulation of NOS and NOX expression.